Product knowledge & term of the month KW05/2026

Lonza Nucleofector™ technology - what is it, what is it used for and what are its benefits?

Lonza Nucleofector technology is a special electroporation process that can be used to introduce nucleic acids (DNA, RNA, siRNA, mRNA, CRISPR components) and proteins into cells very efficiently, right into the cell nucleus.
In contrast to conventional electroporation, the molecules do not just enter the cytoplasm, but reach the site of action directly. This makes nucleofection particularly suitable for cell types that are difficult to transfect.

How it works

• Cell type-specific optimized electrical impulses

• Special nucleofector buffers

• Cell and nuclear membrane become permeable for a short time

• The material reaches the site of action directly (e.g. DNA → cell nucleus)
  
  

Typical applications

• Primary cells (e.g. T cells, B cells, neurons)

• Stem cells (iPSCs, hESCs)

• CRISPR/Cas9 genome editing

• RNA interference (siRNA, miRNA)

• Immune cell engineering (e.g. CAR-T research)
  
  

Advantages at a glance

• High transfection efficiency, even with challenging cell types

• Reproducible, standardized protocols (optimized for specific cell types)

• Widely established and cited in many publications

• The 4D-Nucleofector™ has a modular design and can be expanded as required

4D-Nucleofector™ brochure

All features, modules and workflows at a glance.

4D-Nucleofector on our website:

Nucleofection

A special form of electroporation in which biomolecules are not only introduced into the cytoplasm, but also into the cell nucleus - particularly helpful for primary and stem cells that are difficult to transfect.