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ZymoBIOMICS Microbial Community Standards
Microbiomics has rapidly advanced in recent years, yielding numerous new discoveries. However, the field lacks a comprehensive assessment of the reliability of its methods and protocols. Many researchers have highlighted issues with data reproducibility across labs and the challenges of managing the considerable variability inherent in the complex microbiomics workflow.
NGS microbiomics workflows consist of multiple intricate steps, often complicated by biases and errors occurring throughout. Figure 1 outlines the typical challenges encountered at each stage of the microbiomics workflow. Even freezing, which is considered the gold standard, can potentially suffer from bias caused by freeze-thaw cycles. Read our news to learn how bias can arise in every step of your workflow.
Figure 1. Potential sources of bias or error throughout the entire microbiomics workflow.
At Szabo-Scandic, we offer a comprehensive selection of ZymoBIOMICS® solutions designed to improve reliability and reproducibility throughout the microbiomics workflow. The portfolio includes microbial community standards, sample collection and preservation solutions, DNA/RNA isolation kits, and library preparation products, supporting researchers at multiple stages of microbiome analysis.
ZymoBIOMICS® microbial community standards provide well-defined reference materials that help researchers validate, optimise, and standardise their methods and increase confidence in their results. By incorporating reliable controls into microbiome studies, researchers can better identify potential sources of bias and improve the overall accuracy and reproducibility of their workflows.
Explore the ZymoBIOMICS® solutions available from Szabo-Scandic and find the right products to support your microbiome research.
Microbial Community Standards available
| ZymoBIOIMCS standards | ||||||||
|---|---|---|---|---|---|---|---|---|
| Formats | Microbial cells | Isolated DNA | Microbial cells | Isolated DNA | High microbial load | Low microbial load | Natura material, whole cell | |
| Detects bias in | Complete workflow | Library prep and sequencing | Complete workflow | Library prep and sequencing | Individual samples | Individual samples | Complete workflow | |
| Recommended use | General benchmarking and microbiome profiling control control | Assess detection limit and sensitivity | Absolute quantification of high bacterial load samples e.g. faeces | Absolute quantification of low bacterial load samples e.g. sputum | Method comparison and process control | |||
For long read library prep and sequencing, a high molecular weight DNA standard is available. This isolated DNA standard can be used as a positive control for general benchmarking and microbiome profiling.
